Initial excavations (1935-1941) in San Josecito Cave, Nuevo León, México revealed a rich late Quaternary fossil fauna. The San Josecito Cave fauna was viewed as a single time-related entity despite the lack of 14C dating. Renewed excavations (1990) recovered a series of 14 C samples related to the complex stratigraphy. Six 14C dates show that the recently excavated deposits range in age between 45,000 and 27,000 yr B.P. These ages suggest that the upper deposits, removed in the earlier excavations, date from 27,000 to 11,000 yr B.P.